Sn Rk 202 14 2002

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The CDPK-SnRK (calcium-dependent protein kinase/Snf1-related protein kinase) gene superfamily plays important roles in signaling pathways for disease resistance and various stress responses, as indicated by emerging evidence. In this study, we constructed comparative analyses of gene structure, retention, expansion, whole-genome duplication (WGD) and expression patterns of CDPK-SnRK genes in Brassica rapa and their evolution in plants. A total of 49 BrCPKs, 14 BrCRKs, 3 BrPPCKs, 5 BrPEPRKs, and 56 BrSnRKs were identified in B. All BrCDPK-SnRK proteins had highly conserved kinase domains. By statistical analysis of the number of CDPK-SnRK genes in each species, we found that the expansion of the CDPK-SnRK gene family started from angiosperms. Segmental duplication played a predominant role in CDPK-SnRK gene expansion.

Purcell PC, Smith AM, Halford NG (1998) Antisense expression of a sucrose non-fermenting-1-related protein kinase sequence in potato results in decreased expression of sucrose synthase in tubers and loss of sucrose-inducibility of sucrose synthase transcripts in leaves. Plant J 14:195–202 CrossRef Google Scholar.

Sn rk 202 14 2002 full

The analysis showed that PEPRK was more preferentially retained than other subfamilies and that CPK was retained similarly to SnRK. Among the CPKs and SnRKs, CPKIII and SnRK1 genes were more preferentially retained than other groups. CRK was closest to CPK, which may share a common evolutionary origin. In addition, we identified 196 CPK genes and 252 SnRK genes in 6 species, and their different expansion and evolution types were discovered. Furthermore, the expression of BrCDPK-SnRK genes is dynamic in different tissues as well as in response to abiotic stresses, demonstrating their important roles in development in B. In summary, this study provides genome-wide insight into the evolutionary history and mechanisms of CDPK-SnRK genes following whole-genome triplication in B.

Identification and classification of the CDPK-SnRK superfamily of protein kinases in Brassica rapa and comparative analyses In this study, genome-wide analysis of CDPK-SnRK gene family has been performed on the basis of the completed B. Rapa genome sequence (Wang et al., ). Based on previously reported methods (Harmon et al.,; Hrabak et al., ), the homogeneous candidate CDPK-SnRK genes between Brassica rapa and other species were identified by BLASTP (Supplementary Table ). Subsequently, all candidate protein sequences were subjected to Pfam and SMART analyses. Finally, we identified 49 BrCPKs, 14 BrCRKs, 3 BrPPCKs, 5 BrPEPRKs, and 56 BrSnRKs named according to nomenclature proposed for CDPK-SnRK genes (Supplementary Table ). The urbz sims in the city ps2 ntsc bios download pc. To better understand the expansion and evolutionary history of CDPK-SnRK genes in B. Rapa, genes were also identified in 16 other species representing the major clades of plants.